Aseptic techniques - required practical 6

• What is an aseptic technique?


  Working in sterile conditions to avoid contamination and infection of others.
• Describe and explain 3 aseptic techniques that are used to transfer bacteria onto agar?

  wash hands to remove/kill microbes

  or

  Wear gloves/apron to prevent contamination

  Burning Bunsen close by to create an upward current of air

  Disinfect bench/work on disinfected cloth to kill microbes/prevent contamination

  Lift lid slightly to prevent entry of microbes.
• What are the 3 hazards with the antibiotic practical?


  Disinfectant, Biohazard and Naked flame
• What is the risk and safety precautions for Disinfectant?

  Risk: flammable

  Safety precautions: Keep away from the naked flame

  In emergency: put out fire and seek assistance.

  Low risk.
• What is the risk and safety precautions for Biohazard?

  Risk: contamination and infection

  Safety precautions: use disinfectant, wash hands with soap after dissection, do not incubate at human body temperature, do not open agar plate post incubation.

  In emergency: seek assistance

  The risk is low/medium (depending on the likeliness of bacteria sample used to cause infection)
• What is the Risk and safety precautions for the naked flame?

  Risk: Fire hazard/burns

  Safety precaution: Keep away from flammable materials(goggles and tie up hair)

  In emergency: Put out fire, seek assistance and run burns under cold water immediately.

  The risk is low.
• What is the equation for measuring zones of inhibition?

  pir^2
• How do we know which antibiotic works the best?

  It has the largest inhibition zone.

  The larger the inhibition the more bacteria the antibiotic has killed.

  If the inhibition zone is smaller it means the bacteria is more resistant.
• What are the limitations of the aseptic techniques practical?

  It is time consuming, is difficult growing microorganisms and can lead to infection if the correct safety isn`t followed.