Preparation of the Hemocytometer

• 1st step is before loading your sample, make sure to wipe the hemocytometer using a clean GAUZE PAD, dipped in 70% ISOPROPYL ALCOHOL. Make sure that the hemocytometer is DRY before loading your sample.
• 2nd step is to prepare a WET CHAMBER by placing a damp GAUZE PAD (use WATER to dampen the pad) inside a PETRI DISH.
• 3rd step is after mixing the sample, hold the pipette VERTICALLY with the index or middle finger covering the top of the pipette. Discard the FIRST FOUR DROPS of the mixture onto a piece of gauze.
• 4th step is to place a COVERSLIP onto the counting chamber.
• 5th step is to gently release your index or middle finger from the PIPETTE to allow a few drops of sample to flow.
• 6th step is to place the TIP of the pipette on the EDGE of the RULED AREA of the counting chamber and gradually allow the mixture to seep under the cover glass. Fill this area exactly. Repeat this step on the other side of the counting chamber.
• 7th step is to place the charged hemocytometer inside the WET CHAMBER and cover it. Incubate at ROOM TEMPERATURE at 10 MINUTES.