1st step is after incubation, place the hemocytometer onto the stage of the microscope. Keep the counting chamber at a HORIZONTAL position at all times.
2nd step is to adjust the microscope using the LPO with LOW LIGHT
3rd step is to identify the MARKINGS in the counting chamber.
4th step is in proper focus, WBCs should look like what?
small darks or black dots
With what texture?
grainy or sandy
5th step is to scan the 4 LARGE WBC SQUARES for an even distribution of the cells. If the distribution is uneven, repeat the loading process.
6th step is beginning on the UPPER LEFT LARGE SQUARE, count all WBCs in the four large corner squares and add the results to obtain the total number of cells counted.
7th step is to count the cells on the opposite side of the counting chamber and record the number of cells counted in these 4 large squares. The total should be close to the first count should only have NO MORE THAN OR EQUAL TO 20% difference.
The last step is to get the AVERAGE of the count for the 2 CHAMBERS and compute
