Procedure for four quadrant streak plate
1. In the first quadrant, you will get three (3) loopful from your bacterial suspension and then you streaked in this manner. Turn 45 degrees and then streak
2. Touch the first quadrant 2-3 times
3. Before you proceed to your second quadrant make sure you are going to sterilize the inoculating loop
4. Hit the inoculating loop in between quadrants in order for you to isolate the pure culture and in order for you to prevent contamination
5. Streak first the first quadrant and then hit your inoculating loop
6. Then touch your first quadrant 2-3 times and streak for your second quadrant and then turn it 45 degrees
7. Streak again for your third quadrant. Touch 2-3 times only
8. And then for your fourth quadrant, since this is the quadrant where you will find the pure culture or your individual colonies. You will touch your third quadrant only once