Pavlov rang a bell before giving food to dogs while measuring the salivation of the dogs. The food caused salivation as expected. In this case, the food was the unconditioned stimulus and the salivation was the unconditioned response (these are called unconditioned as they did not need to be learned - salivation is an instinctive response to food). At the start of the study, the bell did not cause salivation and so was the neutral stimulus. After the bell was rung before food was given to the dogs several times, the dogs began to salivate when they heard the bell. At this point, the bell had become a conditioned stimulus and the salivation (in response to the bell) is the conditioned response (these are called conditioned as the stimulus-response connection has been learned rather than being instinctive). Pavlov's research showed that animals learn to associate two stimuli which occur together.