L10-12 quiz

• what type of enzyme is chymotrypsin?


  serine protease
• catalytic triad found in many protease enzymes provides a reactive site site that undergoes both:


  acid-base and covalent catalysis during the cleavage of peptide bonds in the substrate
• which amino acid does sarin and DIPF react with?

  serine
• what types of inhibitors are useful for mapping enzyme active sites?

  • suicide inhibitors
  • affinity labels
  • group-specific reagents
• isozymes are 


  enzymes that have the same function but different properties and are encoded by different genes
• mechanisms for regulating enzyme activity:

  • phosphorylation
  • feedback inhibition
  • proteolytic cleavage
• zymogens are 


  inactive enzymes
• effect of phosphorylation:

  • alter net charge of the protein
  • alter the conformation of the protein
  • activate the enzyme
• if plot of the reaction rate of an enzyme against substrate concentration produces a sigmoidal curve, then the enzyme is said to be an _ enzyme

  allosteric