Ion exchange resin decalcification
1. Layer of resin spread on bottom of container, specimen placed on top
2. Decalcifying agent added, usually 20-30 times volume of tissue
3. Tissue may stay in solution 1-14 days, degree of decalcification measured by physical or X-ray method
4. Used resin can be reactivated by immersing in HCl and washing with water