Can't measure active blood flow as person is dead, Can't measure brain activity as person is dead, No temporal validity, Takes time to do, Unsure of causation as the structure that is abnormal may not be the cause of the behaviour/issues being examined - unsure if changes are a result of neuronal changes after death, Sample size issues - may be difficult to get people to agree to their brain being used in research, Ethical issues of consent - may be carried out on patients with severe psychological deficits.