dna purification practical

• why do white blood cells not need to be crushed before adding detergent

  no cell walls to break open and they are individual cells and not apart of a tissue so there is no separation needed
• why are the white blood cells mixed with detergent in step 1

  disrupts the phospholipid bilayer
• what does protease do 


  breaks down the proteins associated with dna such as histones
• why should you grind leaves

  to break their cell walls
• why should you not use pineapple protease in pineapple juice to extract pure leek dna

  pineapple juice contains pineapple dna

  the ph might be too low
• what is it called when a white layer of DNA forms between the sample and the ethanol

  precipitation
• why is a salt added

  helps dna precipitate
• why is RNAase added

  to remove rna from dna
• what removes rna from dna

  RNAase
• why is ethanol added

  to precipitate the dna
• what does detergent do

  works as an emulsifier

  to disrupt the phospholipid bilayer
• what reaction joins two nucleotides in a polynucleotide

  condensation