Drug test

• Alcohol Rapid Test Strip


  A rapid test for the qualitative detection of Alcohol (ALC) in human saliva
• The distinct color on reactive pad could be observed in the less than 60s, after the reaction pad was wetted with urine specimens with Ethyl Alcohol concentration > 0.2% (20 mg/dL)
• Other alcohols (Methyl, Propanyl & Allyl Alcohol) would develop a similar color on the reactive pad, however, these alcohols are not normally present in human saliva
• Specimen Collection and Preparation

  The oral fluid specimen should be collected using a clean pouch or container
• Avoid contact with skin by wearing gloves and proper laboratory attire
• • This test is a very sensitive to the presence of Alcohol
  • A blue color that is lighter than 0.02% color pad should be interpreted as POSITIVE to the presence of alcohol in saliva, but less than 0.02% relative blood alcohol
• This test is not reusable
• The Alcohol Rapid Test Strip has a sensitivity of 20 mg/dL
• OPI Rapid Test Cassette (Urine)


  A rapid test for the qualitative detection of Opiates in human urine
• During Testing


  1. A urine migrates upward by capillary action

  2. Morphine if present in the urine, specimen below 2,000 ng/mL, will not saturate the binding sites of the antibody in the test

  3. The Morphine conjugate will be captured by an antibody and a visible colored line will show up in the test line region

  4. If the Morphine level exceeds 2,000 ng/mL, the test line will not form because it will saturate all the binding sites of anti-morphine antibodies

  5. A drug-positive urine specimen will not generate a colored line in the test line region because of drug competition

  6. A drug-negative urine specimen or specimen containing a drug concentration less than the cut-off will generate a line in the test line region because of the absence of drug competition
• Procedural Control


  A colored line will always appear at the control line region, indicating that the proper volume of specimen has been added and membrane wicking has occurred
• MDMA Rapid Cassette (Urine)


  A rapid test for the qualitative detection of Methylenedioxy-Methamphetamine (MDMA) in human urine
• During Testing


  1. A urine migrates upward by capillary action

  2. MDMA, if present in the urine specimen < 500 ng/mL will not saturate the binding sites of antibody coated particles in the test

  3. The antibody coated particles will then be captured by immobilized MDMA conjugate and a visible colored line will show up in the region

  4. The colored line will not form if the site of MDMA level exceeds 500 ng/mL, because it will saturate all the binding sites of anti-MDMA antibodies

  5. A drug-positive urine specimen will not generate a colored line in the test region

  6. A drug-negative urine specimen/ a specimen containing a drug concentration less than the cut-off will generate a line in the test region
• The shade of color in the test line region (T) may vary, but it should be considered negative whenever there is even a faint colored line
• The MDMA Rapid Test Cassette has a sensitivity of 500 ng/dL
• MOP Rapid Test Cassette (Urine)


  A rapid test for the qualitive detection of Morphine in human urine
• During Testing


  1. A urine migrates upward by capillary action

  2. Morphine, if present in the urine specimen < 300 ng/mL will not saturate the binding sites of antibody coated particles in the test

  3. The antibody coated particles will then be captured by immobilized Morphine conjugate and a visible colored line will show up in the region

  4. The colored line will not form if the site of Morphine level is at/ above 300 ng/mL, because it will saturate all the binding sites of anti-MDMA antibodies

  5. A drug-positive urine specimen will not generate a colored line in the test region

  6. A drug-negative urine specimen/ a specimen containing a drug concentration less than the cut-off will generate a line in the test region
• Specimen collection and preparation (urine assay)


  1. Urine specimen must be collected in a clean and dry container

  2. Urine collected at any time of the day may be used

  3. Urine specimens exhibiting visible precipitates should be centrifuged, filtered, or allowed to settle to obtain clear specimens for testing
• Positive (+) result


  • One color line appears in the control region (C), no line appears in the test line region (T)
  • Indicates that the drug concentration exceeds the detectable cut-off level
• Negative (-) result


  • Two lines appear, one colored line in the control line (C) and another apparent colored line in the test line region (T)
  • Indicates that the drug concentration is below the detectable cut-off level
• Invalid


  • Control lines fail to appear
  • Insufficient specimen volume/ incorrect procedural techniques are the most likely reasons for control line failure
• Procedure for invalid result

  1. Review the procedure and repeat the test using a new test kit

  2. If the problem persists, discontinue using the lot immediately and contact your local distributor
• The Multi-Drug RapidTest Cassette (Urine) provides only a qualitative, preliminary, analytical result
• A secondary analytical method must be used to obtain a confirmed result
• Gas Chromatography Spectrometry (GC/MS) is the preferred confirmatory method
• Technical or procedural errors, as well as other interfering substances in the urine specimen may cause erroneous results
• Adulterants (bleach/ alum), in the urine specimens may produce erroneous results regardless of analytical method used
• Procedure if adulterants are suspected

  The test should be repeated with another urine specimen
• A (+) result indicates presence of the drug or its metabolites but does not indicate level of intoxication, administration route or concentration in the urine
• A (-) result may not necessarily indicate drug-free urine
• (-) results can be obtained when drug is present but below the cut-off level of the test
• Test does not distinguish between drugs of abuse and other medications
• Negative result


  Indicates that the concentration of the drug is below the detectable level
• Positive result


  Means that the concentration of the drug is above the cut-off level
• The Multi-Drug Rapid Test Cassette has a sensitivity of the cut-off level
• Marijuana (THC)


  • THC (Delta-9-Tetrahydrocannabinol) is the primary active ingredient in Cannabis (Marijuana)
  • When smoked or orally administered, THC produces euphoric effects
  • Users have impaired short-term memory and slowed learning
  • May also experience transient episodes of confusion and anxiety
• Long term effect of marijuana

  • Relatively, heavy use may be associated with behavioral disorders
• Peak effect of marijuana

  • Administered by smoking occurs in 20-30 minutes and the duration is 90-120 minutes after one cigarette
• Elevated levels of urinary metabolites of marijuana


  • Found within hours of exposure and remain detectable for 3-10 days after smoking
  • The main metabolite excreted in the urine is 11-Nor-Delta-9-Tetrahydrocannabinol-9-Carboxylic Acid (THC-COOH)
• Methamphetamine (MET)


  • An addictive stimulant drug that strongly activates certain systems in the brain
  • Closely related to chemically Amphetamine, but the central nervous system effects of methamphetamine are greater
  • Made in illegal laboratories and has a high potential for abuse and dependance
  • The drug can be taken orally, injected, or inhaled