Hemoflagellates

• What genus is included in hemoflagellates?

  Leishmania and trypanosoma
• Hemoflagellates may infect what organs?

  blood, lymph nodes, muscles, and cells of the reticuloendothelial system
• What are the 4 morphological stages of hemoflagellates?

  Amastigote, promastigote, epimastigote, and trypomastigote
• Other name for amastigote?

  leishmanial form
• Other name of promastigote?

  leptomonal form
• Other name for epimastigote?

  Crithidial form
• Other name for trypomastigote?

  trypanosomal form
• Complete the table:

  A) Host

  B) Vector

  C) Vector

  D) Vector and Host

  
• Amastigote: roundish to oval in shape
• Amastigote: ECCENTRIC nucleus
• Amastigote: DOT-LIKE blepharoplast
• Amastigote: small parabasal body
• Amastigote: presence of axoneme
• Amastigote has no what?

  flagellum
• Label the following:

  A) Nucleus

  B) Axoneme

  C) Blepharoplast

  D) Parabasal body

  E) Kinetoplast

  
• What is this?

  Leishmania spp. amastigote

  
• Promastigote: Slender in shape
• Promastigote: SINGE nucleus
• Promastigote: anterior kinetoplast
• Promastigote: anterior flagellum
• Promastigote: also has a presence of axonemes
• Promastigotes lack what?

  undulating membrane
• What is the infective stage of Leishmania spp?


  Promastigotes
• Label the following:

  A) Axoneme

  B) Flagellum

  C) Blepharoplast

  D) Parabasal body

  E) Nucleus

  F) Kinetoplast

  
• What is this?

  Leishmania spp. promastigotes

  
• Epimastigote: Longer & slightly longer
• Epimastigote: POSTERIOR nucleus
• Epimastigote: 1/2 undulating membrane
• Epimastigote: also has anterior flagellum
• Label the following:

  A) Nucleus

  B) Parabasal body

  C) Blepharoplast

  D) Undulating membrane

  E) Flagellum

  F) Kinetoplast

  
• Trypomastigotes are of what shapes?

  C, S, U
• Trypomastigote: full body length undulating membrane
• Trypomastigote: POSTERIOR kinetoplast
• Trypomastigote: meron ring anterior flagellum
• What is the infective stage of Trypanosoma?

  Trypomastigotes
• Label the following:

  A) Nucleus

  B) Undulating membrane

  C) Axoneme

  D) Blepharoplast

  E) Parabasal body

  F) Kinetoplast

  G) Flagellum

  
• What is this?

  Trypanosoma brucei species trypomastigote

  
• What is this?

  Trypanosoma brucei spp trypomastigotes

  
• What are the diagnosis for Hemoflagellates?

  Direct Examination using GIEMSA STAIN and CULTURE
• What medium is used for culturing hemoflagellates?

  Novy-MacNeal-Nicolle medium