Microscopes

• Image Size= Actual Size X Magnification
• 1 km= 1000m

  1m= 100cm

  1cm= 10mm

  1mm= 1000 micrometers

  1 micrometer= 1000 nanometers
• Light Microscopes:

  • Weaker magnification
  • Samples can be living or dead
  • Image is in 2D
  • Sample needs to be stained to show features
  • Needs a very thin sample
  • Colored
  • Used in schools
• Electron Microscopes:

  • Stronger magnification
  • Image can be 2-D or 3-D
  • Needs a dead sample
  • Expensive
  • Samples need to be coated in chemicals or gold
  • Samples place in a vacuum
• Parts of a microscope:

  A) Eyepiece

  B) Coarse focus

  C) Coarse focus

  D) Mirror or light source

  E) Stage

  F) High power objective lens

  G) Low power Objective lens

  H) Stage Clips

  I) Diaphragm

  
• Magnification= Size of a sample under a microscope
• Resolution= Amount of detail on the sample
• Total Magnification= Objective Magnification X Eyepiece Magnification
• Why do we use the lowest magnification first when using microscopes?

  it provides a wider field of view, making it easier to locate and center the specimen.