Special Blood Bank Collection

• A red cell suspension is a common reagent used in performing what type of procedures?

  Serological procedures
• A red cell suspension is primarily used in detection of RED CELL ANTIGENS and in assessing the binding strength of an ANTIBODY
• What are the different concentrations of a red cell suspension?

  0.8%, 3%, 5%, and 10%
• What is the MOST COMMON CONC. of a red cell suspension?

  3%
• What is the WASH SOLUTION and DILUENT of a red cell suspension?

  0.9% NSS
• The function of NSS is to preserve the what of RBCs?

  morphology
• NSS is what type of solution?

  Isotonic
• Solvent: NSS
• Solute: RBC
• What is color of the suspension of a red cell?

  cherry or tomato red
• IgG-coated RBCs are also known as what?

  Coomb's control cells or "O" check cells
• What blood type is used for Red cell suspension?

  O
• IgG-coated RBCs are called O check cells because it ensures that what are TRUE NEGATIVES?

  antiglobulin test
• It also ensures that the AHG is what to ensure the viability of AGT and AHG reagents?

  not inactivated
• IgG-coated RBSs are red cell suspensions of what concentration of a single donor of what group of RBC?

  0.8% to 5%, O
• In IgG-coated RBC, RBCs are washed to remove all blood group ANTIBODIES, coated with IgG, and resuspended in a DILUENT.
• Treated RBCs may WEAKEN or DESTROY unwanted or untested RBCs ANTIGENS while enhancing the expression or reactivity of antigens of interest.
• What are the reagents used in TREATING RBCs?

  PAPAIN and FICIN or DITHIOTHREITOL and 2-aminoethylisothiuronium bromide
• AET: an organic solvent
• What removes INTERFERENCES in TREATED RBCs?

  Enzymes