Factors affecting enzyme activity
1. As temperature increases to the optimum, the kinetic energy of the enzyme and substrate increases, causing more collisions between the enzyme and substrate
2. An increase in temperature beyond the optimum causes the enzyme's active site to become denatured (The active site loses its important shape and can no longer form enzyme-substrate complexes, leading to a decrease in enzyme activity)
3. Deviating from the optimum pH (too high/low) causes the enzyme's active site to become denatured and the active site loses its important shape
4. The higher the enzyme concentration, the more enzymes there are to form enzymes-substrate complexes, leading to an increase in enzyme activity
5. Enzyme activity plateaus as there are not enough substrate molecules to react with the extra enzymes