CULTURE

• Culture


  The growth or crop of microorganisms obtained in a culture after its incubation period
• Culture medium


  Any material where microorganisms may thrive for their nourishment & reproduction
• Incubation Period


  The time needed for the microorganisms to adapt, grow & multiply in its new environment
• Types of Culture


  • Contaminate Culture
  • Mixed Culture
  • Pure Culture
• Inoculum


  The fish-out colony hanging on the wire loop, cotton swabs etc. that is ready for transfer to another culture medium for cultivation and further isolation
• Colony


  Group of microorganisms growing together characteristically in a culture medium
• Types of Culture Media According to Physical State

  • Solid/semisolid
  • Liquid Media
• Solid/semisolid culture media


  • Nutrient Agar
  • BAP
  • EMB
• Liquid culture media


  • Nutrient Broth
• Types of Culture According to Use

  • Simple media
  • Enrich media
  • Selective media
  • Differential media
  • Transport media
• Simple Media


  Used as a base in making of more complex media, with beef extract, Peptone, agar, water
• Simple Media


  • Nutrient Agar
• Enriched Media


  With special added nutrient to promote growth
• Enriched Media

  • Blood Agar Plate (BAP)
  • Chocolate Agar Plate (CAP)
  • Selenite F. Broth
• Selective media


  Discourage the growth of other microorganism
• Selective media

  • Mannitol Salt Agar (MSA)
  • Bordet-Gengau medium
  • Cetrimide Agar
  • Thayer- Martin Agar
  • Lowenstein- Jensen Medium
  • Saboraud's Dextrose Agar (SDA)
  • Mueller- Hilton Agar (MHA)
• Differential Media

  • Eosin Methylene Blue (EMB)
  • Mac Conkey Agar (MCA)
  • Salmonella- Shigella Agar (SSA)
• Transport Media


  Protect pathogens present in the specimen
• Transport Media

  • Stuart's Transport Medium
  • Cetrimide Agar
  • Thayer- Martin Agar
  • Lowenstein- Jensen Medium
  • Mannitol Salt Agar
  • Bordet- Gengou Medium
  • Blood Agar Plate
  • Chocolate Agar Plate
  • Selenite F Broth
• Transport Media

  1. Stuart’s Transport Medium. 2. Cetrimide Agar - Pseudomonas Aeruginosa. 3. Thayer- Martin Agar- genus Neisseria 4. Lowenstein- Jensen Medium
  2. (LJ Medium)- Mycobacteria
  3. 5. MSA- differential Agar for various species of staphylococci ( it has a different appearance in MSA.)
  4. 6. Bordet- Gengou Medium- Bordetella Pertussis
  5. 7. BAP- hemolytic streptococci
  6. 8. CAP- Haemophilus species.
  7. 9. CAP with CO₂- Neisseria Meningitidis Neisseria Gonorrhoeae
  8. 10. Selenite F Broth- Salmonella, Shigellae