Substrate Concentration

• Substrate Concentration


  The amount of substrate available for the enzyme to bind to
• Enzyme Activity: Substrate Concentration

  1. The greater the substrate concentration, the higher the rate of reaction

  2. As the number of substrate molecules increases, the likelihood of enzyme-substrate complex formation increases

  3. If the enzyme concentration remains fixed but the amount of substrate is increased

  4. Past a certain point, all available active sites eventually become saturated and any further increase in substrate concentration will not increase the reaction rate

  5. When the active sites of the enzymes are all full, any substrate molecules that are added have nowhere to bind in order to form an enzyme-substrate complex
• Substrate concentration increases


  Reaction rate increases linearly
• Substrate concentration increases past active site saturation point


  Reaction rate plateaus
• The rate of reaction follows a pattern when the amount of substrate concentration increases