Enzyme Concentration

• Enzyme Activity: Enzyme Concentration

  1. Enzyme concentration affects the rate of reaction

  2. The higher the enzyme concentration in a reaction mixture, the greater the number of active sites available and the greater the likelihood of enzyme-substrate complex formation

  3. As long as there is sufficient substrate available, the initial rate of reaction increases linearly with enzyme concentration

  4. If the amount of substrate is limited, at a certain point any further increase in enzyme concentration will not increase the reaction rate as the amount of substrate becomes a limiting factor
• Enzyme concentration


  Affects the rate of reaction
• Higher enzyme concentration


  Greater number of active sites available and greater likelihood of enzyme-substrate complex formation
• Sufficient substrate available


  Initial rate of reaction increases linearly with enzyme concentration
• Limited substrate


  Further increase in enzyme concentration will not increase the reaction rate
• The amount of substrate becomes a limiting factor