Retrotransposons

• interspersed retro transposable elements


  sines and lines
• do not have inverted terminal repeats

  lines and sines
• found in almost all eukaryotes

  sines and lines
• autonomous retroelements


  lines
• dependants


  sines
• encodes a rna binding protein

  orf 2
• encodes a protein with endonuclease and reverse transcriptase activity

  orf 2
• can be seen in poly a retrotransposon

  5' and 3' utr
• adjacent to 3' utr

  T rich region
• encodes a protein with endo and reverse transcriptase activity


  lines
• depend on reverse transcriptase and endonuclease functions encoded by partner lines

  sines
• ensures that proteins bind to line with high affinity


  specific sequence
• transcribe integrated line sequences

  rna polymerase
• function of lines and sines

  mutagenic

  epigenetic silencing

  enhancer
• copy and paste themselves into different genomic locations by converting rna back into dna through the reverse transcription process using an rna transposition intermediate


  retro transposon
• virus like retrotransposon


  ltr
• poly a retrotransposon


  non-ltr
• ltr integration to genome

  cDNA

  integrase

  target dna