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biology unit 1 higher
1.1-cells and movement across membranes
using a microscope
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A convenient source of large cells to look at is an
onion
to make a slide from onion cells it is best to use the
membrane between layers
to look at the membrane of an onion we should
put some on a glass slide
we should stain onion cell with
iodine solution
before looking at a slide, we put on a
cover slip
we look at the prepared slide with a
microscope
we start with the
smallest
objective lens and then gradually
increase
the magnification
we start by making the gap between objective lens and slide as
small
as possible, while looking at it
from the side
when looking through the
eyepiece
, we can adjust the
focus
knob until the image becomes
clearer
if the image is blurry, we need to move the
stage
up or down so that the specimen comes into
focus
higher power lenses have
smaller
fields of view
to see more detail, we use
higher
power lenses