Pipette use

• True - Avoid using the lowest capacity of a pipette
• False - after using, it is okay to not return the settings to max capacity
• Steps in using pipette (in order)

  1. setting the volume
  2. Aspirating a liquid
• Steps in aspirating a liquid

  1. push the plunger up to 1st stop
  2. Immerse the disposable tip of the micro-pipette while still holding down the plunger button
  3. Release the plunger to create a partial vacuum
  4. Place the tip to the vessel
  5. Push to first stop
  6. Push plunger to the second stop to empty the tip completely
• Asepsis - absence of pathogens
• Types of Aseptic technique

  • sterilization
  • disinfection
• sterilization - complete destruction of all living organisms including cell viruses
• Sterile - this term means "devoid life"
• Methods of Sterilization

  • dry heat
  • autoclave
  • gas
  • chemicals
  • certain types of radiation
• Disinfection - destruction/removal of pathogens from objects by physical or chemical methods
• Pasteurization - a disinfectant method using liquid
• Antiseptics - solutions to disinfect skin and other living tissues
• Heat - method of sterilization and disinfection that is most practical, efficient, inexpensive that can withstand high temperature
• Types of heat sterilization method

  • incineration
  • autoclave
• Incineration - most common treatment of biomedical waste; 870-980 degrees Celsius
• Autoclave - usually used in laboratories; enzymes and structural proteins are denatured at 15 psi; 121 degrees celsius for 20 minutes at 15 psi