topic 3: protein quantitative analysis

• True - to create peptide bonds, there is a series of condensation and dehydration
• Human C-reactive Protein - is an indicator of inflammation
• Bradford Assay - measures protein content of a solution using colorimetric method
• Coomassie Brilliant Blue (CBB) - is a dye used in Bradford Assay that is binding with the protein
• True - the intense the color blue means the higher protein concentration
• Kjeldahl method - is another protein assay that indirectly measure protein because it is used to measure total nitrogen
• UV-Vis Spectrophometry - only works in purified proteins wherein absorbance of sample is measured at 280 nm
• Biuret method - it results the product to form a purple color which absorbs at 540 nm
• Lowry Assay - an extension of Biuret Method that involves redox reaction of copper ions; it produces blue green solution
• Bicinchoninic Acid Assay - same principle with Lowry method but copper ions react with BCA to produce a colored product