To isolate DNA From plant tissue

• Why is the plant tissue chopped?

  to increase surface area
• Why is washing up liquid added?

  To break down the cell membrane
• Why is salt added?


  So it can clump together
• Why is the test tube heated in A water bath? Only for 15 minutes


  To denature the enzyme
• Why is it cooled?

  To slow the break down of DNA.
• Why is it blended for 3-5 seconds?

  To break down the cell wall
• Why is it poured through a filter paper?

  To collect the DNA
• Why is protease added?


  To break down proteins surrounding DNA.
• Why is ice cold ethanol added?

  As DNA is Insoluble in ice cold alcohol and so it precipitates
• What are the results?

  white strands of DNA are seen in the alcohol