Bile is made in the liver and stored in the gall bladder. It is alkaline to neutralise hydrochloric acid from the stomach. It also emulsifies fat to form small droplets which increases the surface area. The alkaline conditions and large surface area increase the rate of fat breakdown by lipase.
hthe lock is the enzyme and the key is the substrate. Only the correctly sized key (substrate) fits into the key hole (active site) of the lock (enzyme).
1. At low temperatures there is less kinetic energy so less collisions between enzyme and substrate, leading to a low rate of reaction. 2. At the optimum (best) temperature there is more kinetic energy so more collisions between enzyme and substrate leading to a high rate of reaction. 3. Above the optimum temperature the bond that hold the enzyme together break and the 3D shape is lost. The active site is no longer the same shape so the rate of reaction is lowers.