Describe how you could investigate the effect of pH on the rate of amylase activity
1. Put a drop of iodine solution in each well of a spotting tile.
2. Place a Bunsen burner on a heat proof mat with a tripod and gauze over the Bunsen burner. Put a beaker of water on top f the tripod and heat the water until its 35 degrees using a thermometer. (keep temperature consistent throughout)
3. Use a syringe to add 1cm(3) of amylase solution and 1cm(3) of buffer solution with a pH of 5 to a boiling tube. Put the tube in the beaker of water wait 5 minutes.
4. Use a different syringe to add 5cm(3) of starch solution to the boiling tube.
5. Immediately mix the contents of the boiling tube and start the stopwatch.
6. Use continuous sampling to record how long it takes for the amylase to break down all the starch. To do this, use a dropping pipette to take a fresh sample from the boiling tube every 30 seconds and put a drop into a well. When the iodine solution remains browny-orange, starch is no longer present.
7. Repeat the whole experiment with buffer solutions of different pH value.