Culture micropara

• Culture


  The growth or crop of microorganisms obtained in a culture medium after its incubation period
• Culture Medium


  Any material where microorganisms may thrive for their nourishment and reproduction
• Incubation Period


  The time needed to let previously inoculated culture media to show a distinct colony or colonies within a desired temperature. It is also the time needed for the microorganisms to adapt grow and multiply in the new environment
• Colony


  A group of microorganisms growing together characteristically in a culture medium
• Inoculum


  The fished-out colony hanging on the wire loop, cotton swabs, etc. that is ready for transfer to another culture medium for cultivation and or further isolation
• Types of Culture


  • Contaminated Culture
  • Mixed Culture
  • Pure Culture
• According to physical state

  • Liquid
  • Solid
  • Semi-solid
• Agar


  A polysaccharide derivative of seaweed; dissolves in water at boiling point and sets at 38 degrees Celsius; a solidifying agent
• Gelatin


  Used as a diagnostic medium, solidifies at 25 degrees Celsius
• Albumin


  Coagulates upon heating
• According to application/function/use


  • Simple/ordinary/basal
  • Enriched
  • Selective
  • Differential
• Selective media


  • Mannitol Salt Agar
  • Bordet-Gengou Medium
  • Cetrimide Agar
  • Thayer Martin Agar
  • Lowenstein Jensen Medium
  • Saboraud's Dextrose Agar
  • Mueller-Hinton Agar
• Differential media


  • Eosin Methylene Blue
  • Mac Conkey Agar
  • Salmonella Shigella Agar
• Transport Media


  Have been devised to protect pathogens present in clinical specimens that might not otherwise survive or that might be overgrown by non-pathogens during the transport of the specimen from the patient to the laboratory
• Transport Media


  • Stuart's Transport Medium
• According to form or distribution

  • Plated Medium
  • Tubed Medium
• Cultivation


  The growing of bacteria in a culture medium
• Aerobic cultivation


  • Use of suitable containers
  • Constant shaking of liquid medium to introduce oxygen
  • Forcing air through the medium by pressure or suction
• Anaerobic cultivation


  • Addition of reducing agent such as sodium thioglycollate
  • Mechanical removal of oxygen from an enclosed vessel by pumping the air out and replacing it with nitrogen, hydrogen or H2-CO2 mixture (gaspak)
  • Chemical reaction within an enclosed vessel containing the inoculated medium to combine free O2 into a compound by combustion
• Incubator


  An apparatus in which constant degree of temperature (37degrees Celsius) is maintained for purposes of growing cultures of bacteria