Enzymes are used to isolate the required human gene which is then replicated.
This gene is inserted into a vector
which is a bacterial plasmid.
The modified plasmids also include a gene
for antibiotic resistance.
The plasmid inserts the genes into the target bacterial cells.
An antibiotic is added which kills any bacteria
which have not taken up the plasmid.
The bacteria that survive will be able to also make insulin.
They are grown in a fermenter and the insulin is harvested.