Method of Investigation into factors affecting enzyme action
1. Measure 10cm3 of 1% starch solution into a test tube
2. Measure 2cm3 of 10% amylase solution into a second test tube
3. Place both tubes into a water bath set at 20ºC for 3 minutes
4. Place a drop of iodine in six wells of a spotting tile
5. Remove both test tubes from the water bath, pour the amylase into the starch/iodine solution and start the stopwatch
6. Immediately, use the dropping pipette to place one drop of the mixture onto the first drop of iodine, record the colour of the solution
7. Repeat step 6 every minute for five minutes
8. Repeat steps 1-7 at 30ºC, 40ºC, 50ºC, 60ºC