RP14

• Gel electrophoresis


  Used to separate DNA fragments of different sizes
• Gel electrophoresis process


  1. DNA fragments (cut with restriction endonuclease enzymes) loaded into wells in agarose gel

  2. Current applied

  3. DNA moves towards anode

  4. Different DNA fragments move at different rates

  5. Bands created at different heights
• Gel electrophoresis


  • Used as part of DNA profiling
• There are significant risks associated with this practical and the equipment involved is expensive
• It is likely you'll use a simulation of this practical instead of the actual process
• See the video from Addgene for a detailed explanation and demonstration of the method