Venous Blood Samples

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Created by
Thatus Licudan

Cards (8)

  • Venous Blood Samples
    • Knott's concentration technique
    • Membrane filtration method
  • Knott's Concentration Method
    • Designed to concentrate blood specimens suspected of containing low numbers of microfilariae.
  • Knott's Concentration Method
    • 1 ml venous blood + 2ml 10% formalin in a centrifuge = mixed then spin for 1 minute at 500 x g
  • Knott's Concentration Method
    • Supernatant is discarded and sediment is smeared and stained with Giemsa.
  • Membrane Filtration Method
    • 1 ml of fresh/ anticoagulated blood is drawn up into a syringe and lysed by adding 10ml of distilled water. It is then passed through the Swinney filter.
  • Membrane Filtration Method
    • The filter is removed, placed on a slide, stained with dilute Giemsa stain, and examined under LPO for microfilariae.
  • Membrane Filtration Method
    • Millipore and nucleopore membrane filters are available in this purpose. The latter being more sensitive as it can screen larger volumes of blood.
  • Membrane Filtration Method
    • The most sensitive method of detecting microfilariae.