Patt

• This microscope uses reflected light instead of transmitted light used in the ordinary light microscope thus prevents light from falling directly on the objective lens. The resulting microorganisms appear brightly stained against a dark background

  Dark-field microscope
• The patient have Mycoplasma pneumoniae infection (Walking pneumonia). You want see the presence under a fluorescent microscope. What is the best dye to use?

  acridine
• A patient is suspected having infected by a tubercle bacilli. What is the best fluorochromic dye you will use?

  auramine-rhodamine
• The property by which some atoms absorb light at a particular wavelength and subsequently emit a light of a longer wavelength

  fluorescence
• Part of a microscope that contains a number of leaves that the operator may open or close to increase/decrease the   amount of light illuminating the object

  diaphragm
• What is the magnification of a light microscope when you use you high power objective (HPO)?

  400x
• A key component of a microscope that maintains the extent to which detail in the magnified object could be seen.

  resolution
• Electron microscope, except


  Uses lenses
• A Gram stain is an example of a

  differential stain
• The best staining technique for a capsulated bacteria

  negative staning
• Cationic dye, except.


  eosin
• A method or preparation of smear ideally used for tissue, bone marrow aspirate, or other aspirated sample.

  squash method
• The step in Gram staining where gram-positive from gram-negative cells can already be differentiated.

  decolorization