1. Autotrophs use the energy of sunlight to 'fix' carbon dioxide, turning its carbon into sugars and other organic molecules
2. The Calvin cycle is where CO₂ is freed, by the enzyme Rubisco, which carboxylates RuBP
3. Terrestrial plants use gaseous CO₂ directly from the air
4. Aquatic organisms use CO₂ dissolved in water
5. As much CO₂ is fixed from ocean microorganisms, as from terrestrial plants