PROCESS

• WHAT DOES ELISA STAND FOR?

  enzyme-linked immunosorbent assay
• PROCESS OF THE ELISA TEST?

  1. Add test sample from patient to beaker base
  2. Wash to remove unbound sample
  3. Add antibody (complementary in shape to test antigen)
  4. Wash to remove unbound antibody
  5. Add second antibody (complementary in shape to first antibody with an enzyme attached)
  6. Second antibody binds to first
  7. Rinse unbound antigen off
  8. Substrate for enzyme (colourless) is added
  9. Produces coloured products in enzyme presence
• WHAT INDICATES AN ANTIGEN PRESENCE IN THE ELISA TEST?

  presence of colour
• WHAT INDICITATES ANTIGEN QUANTITY IN ELISA TEST?

  intensity of colour