Histo lab

• Clearing is the process whereby alcohol or a dehydrating agent is removed from the tissue and replaced with a substance that will dissolve the wax with which the tissue is to be impregnated, such as paraffin.
• Clearing is also known as dehydration because it involves removing water from the tissue.
• Clearing is the first stage in the histopathology laboratory process.
• Clearing is necessary to remove a substantial amount of fat from the tissue.
• Clearing is a crucial step in the histopathology laboratory process.
• Clearing is a process whereby alcohol or a dehydrating agent is removed from the tissue and replaced with a substance that will dissolve the wax with which the tissue is to be impregnated, such as paraffin.
• Base-sledge Microtome is a type of microtome that a histotechnician wanted to increase the hardness of paraffin wax.
• Stearic Acid should be added to the paraffin wax to get what the histotechnician wants.
• Wet celloidin is a type of Epoxy based on Bisphenol A.
• When using an exchange resin, the frequency of changing the decalcifying agent is after 2 weeks.
• Araldite is a paraffin wax substitute with a melting point of 46-48°C.
• Both are correct
• Ester wax is the wax to be used for cataract specimens.
• Bioloid is a type of microtome best for ophthalmology.
• Decalcification using ACIDS takes days
• In a rocking microtome, the knife is static and the block moves in an arc-like motion.
• Fluid transfer processor is a method of celloidin that is okay for bones.
• Celloidin is a type of automatic processing wherein the processor is completely closed and the container is periodically filled with a particular fluid.
• In tissue elements are stained in a definite sequence in regressive staining.
• There is no washing or decolorizing step in regressive staining.
• Iron in Weigert's hematoxylin is an example of progressive staining.
• In vital staining, selective staining of living cell constituents is demonstrated, demonstrating cytoplasmic structures by phagocytosis of the dye particle.
• Compound haematoxylin has almost no staining capability unless a mordant is used to enhance its staining capability.
• Examples of suravital stains include Neutral red, Nile blue, Janus Green (mitochondria), and Thionine.
• Potassium alum is used in Ehrlich's hematoxylin.
• In metachromatic staining, specific dyes are used which differentiate particular substances by staining them with a color that is different from that of the stain itself.
• Tryphan blue is an example of a reticuloendothelial system stain.
• Hematoxylin is extracted from the bark of a tree mainly seen in the Campeche state of Mexico, known as Hematoxylin Campechianum or Haematoxylum Campechianum.
• In intravital staining, the dye is injected into any part of the animal body.
• In suravital staining, stains are used that enter and stain living cells.
• Methylene blue is used in indirect staining.
• Ligand dyes consist of dye and a metal ion, also known as metallochrome.
• Auxochrome is a component of a dye that helps to intensify the light and augments more free electrons in the chromophore groups.
• The hot plate is used for deparaffinization/drying sections, with a temperature of 45 - 55 degrees Celsius for 30 - 45 mins.
• The blower-type electric slide dryer is used for deparaffinization/drying sections, with a temperature of 50 - 55 degrees Celsius for 20 - 30 mins.
• Anionic dyes are dyes that carry negative charge and migrate towards the anode.
• The purpose of deparaffinization/drying sections is to remove the paraffin.
• The wax oven is used for deparaffinization/drying sections, with a temperature of 56 - 60 degrees Celsius for deparaffinization and 2 hours for drying.
• Chromophore is a component of a dye that absorbs light and imparts colour to the stain.
• Direct staining is the process of giving color to the sections by using aqueous or alcoholic dye solutions, using only one dye.