unit 2

• recombinant dna technology


  1 - DNA from the donor organism is extracted

  2 - a restriction endonuclease enzyme is used to cut open a DNA fragment containing the required genes from the donor DNA at a restriction site leaving sticky ends

  3 - the same restriction endonuclease is used to cut the vector at restriction sites leaving sticky ends

  4 - DNA ligase is used to seal the DNA fragments into the vector to form recombinant DNA by sealing the complementary sticky ends together
• recombinant dna technology 2


  5 - the recombinant plasmids are inserted into host cells lacking plasmids

  6 - transformed host cells which contain the recombinant vectors are selected and cultured

  7 - transformed cells then translate and transcribe DNA producing the protein product which can then be extracted