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genbio 2
GB2 (1st quarter)
central dogma
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Cards (19)
How did we know that genes are composed of DNA
Hershey-Chase experiment
a principle in molecular biology that describes the relationship between the four nitrogenous bases that make up DNA
Chargaff's rule
how many percent is adenine and thymine in dna
31%
Guanine and Cytosine, how many percent
19%
what does ATCG stands for
Adenine Thymine Cytosine Guanine
Showed DNA is a double helix. It shows some portion of the helix is repeated many times
Franklins X-Ray Diffraction Data
Model of DNA out of wire
The deoxyribose sugar-phosphate backbone make up the sides of the ladder.
The nitrogenous bases make up the rungs of the ladder.
The Watson and Crick Model
the process of making an identical copy of the parent cell’s DNA.
DNA replication
proper order of chargaff's rule
ATCG
Thymine is replaced to what in RNA
Uracil
1st: It opens up the DNA at the replication fork.
Helicase
2nd: it coat the DNA around the replication fork to prevent rewinding of the DNA.
Single-strand binding proteins
3rd: works at the region ahead of the replication fork to prevent supercoiling.
Topoisomerase
4th: synthesizes RNA primers complementary to the DNA strand.
Primase
5th: It extends the primers, adding on to the 3' end, to make the bulk of the new DNA.
DNA polymerase III
6th: They are removed and replaced with DNA by DNA polymerase I.
RNA Primers
7th: The gaps of DNA fragments are then sealed by
DNA ligase
It has Single strand and Sugar: ribose
Ribonucleic acid(RNA)
Produced in the nucleus; where DNA genes are transcribed
Messenger RNA (mRNA)
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