electron transport

• Chemiosmosis

  electrons generate proton gradient

  uses ATPase backwards to generate ATP

  alpha proteobacteria
• Proton Motive Force

  create proton gradient

  oxidizes NADH NADPH FADH2

  flow through F1F0 ATPase

  2 H+ = 1 ATP
• Escherichia Coli Electron Transport

  use: NADH and FADH2

  at least 5 cytochromes; based on PO2

  TEA: oxygen

  inefficient: 1.3 P/O
• Paracoccus Denitrificans

  heterotrophic or autotrophic

  use: H2, CO2, NO2

  TEA: NO2 (reduced to N2)
• Studying ETC

  P/O ratio

  proton motive force: uncouplers

  molecules inhibiting ATPase
• Uncouplers (ionophores)


  inhibit electron transport and phosphorylation: inhibit ATP synthesis