How many times larger an image is than in real life
Magnification
The degree of detail that can be seen (the minimum distance between two separate points can be seen clearly)
Resolution
Most cells are too small to be measured by the international SI unit (metres)- therefore they are mostly measured with micrometres (μm)
Some biological structures are so small and have to be measured by nanometres (nm)
Stage Micrometre: Scale on a microscope slide which is divided into 100 small divisions (1-10/10-100); each division = 0.1mm (10mm long)
Eyepiece Graticule: Scale inside an eyepiece lens that fits into the top of the microscope, you can use it to measure the sample and calibrate to calculate object size
Calibration: Divide the value of the micrometer divisions in µm by the number of graticule divisions that were equivalent to get the real value of the graticule divisions
Objective Magnification - Each small division equals (in mm) - Each small division equals (in microns)
