MICROSCOPE (micropara lab)

• defined as the type of microscope that has more than one lens.

  Compound Microscope
• a convex lens with a small focal length.

  Simple Microscope
• combination of lenses and two optical parts known as an objective lens and an eyepiece or ocular lens.

  Compound Microscope
• type of microscope in which the source of illumination is the

  beam of accelerated electrons.

  Electron Microscope
• a special type of microscope with a high resolution of images as

  the images can be magnified in nanometers.

  Electron Microscope
• defined as a type of microscope that provides a

  three-dimensional view of a specimen.It is also known as a dissecting

  microscope.


  Streo Microscope
• there are separate objective lenses and eyepiece such that there are two separate optical paths for each eye.

  Stereo Microscope
• type of microscope that finds applications in industries where

  the examination of the specimen is done at the nanoscale levels.

  Scanning Probe Microscope
• The study of a specimen’s properties, its reaction time and its behavior when stimulated can be done with the help of a?

  Scanning Probe Microscope
• This part is where the observer looks through to see the magnified image of a sample.

  Eyepiece
• It can magnify the sample up to 10x

  its actual size.It is also called the ocular lens


  Eyepiece
• It holds the objective lenses. It can be rotated to shift from the scanner lens to the oil immersion objective lens.

  Revolving Nosepiece
• These lenses are responsible for magnifying the image of the

  object several times larger than its actual size.

  Objective lens
• It has 4x magnifying power

  Scanner lens
• it has 10x magnifying power.

  Low-power objective (LPO)
• It has 40x magnifying power.

  High power objective (HPO)
• It controls the amount of light that will reach the sample.

  Iris Diaphgram
• It focuses the light on the specimen.

  Condenser
• It reflects light from the light source to the sample.

  Mirror
• It supports the eyepiece and connects it to the revolving nosepiece

  Body Tube
• it is the handle where the user can carry the microscope from one place to another.

  Arm
• It is the the bottom of the microscope that acts as a support.

  Base
• This is a small platform where the glass slide is mounted.

  Stage
• This is a part of clips that secures the slide on the stage

  Stage Clips
• It can move the stage up and down to adjust the distance of

  the sample to be observed. This knob is used when observing

  under LPO.

  Coarse Adjustment Knob
• It is used to focus the sample even more. This knob is used

  when observing under the HPO

  Fine Adjustment Knob
• is an instrument that can be used to observe small objects, even cells.

  Microscope
• Study of organisms that are so small they cannot be seen

  with the naked eye.

  Microbiology
• from the greek word "mikros" means

  small
• from the greek word "bios" means

  life
• from the greek word "logia or logos" means

  study of
• PREPARING THE SLIDES: Put a small amount of water/oil on the slides

  Step 1
• SLIDE PREPARATION: Place the coverslip at a 45 degree angle on top of the specimen. Mount carefully

  Step 3
• SLIDE PREPARATION: Place the specimen using the forceps

  Step 2
• use of colored dyes/ stains to make cells and/or cellular structures visible or to produce contrast between different types of cell/cellular components that enhances the contrast of a microscopic image.

  Staining
• colored organic compounds(salts) used for staining cells, tissues, microorganisms,

  Stains
• Stain Contain ions which impart them color, these ions are called

  Chromophore
• If chromophore is positive ion

  basic stain
• chromophore is negative

  acidic stain
• commonly referred to as colored molecules for this reason

  CHROMOPHORE