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MICROBIOLOGY AND PARASITOLOGY LAB
CULTURE MEDIA
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Mark Anthony Ronquillo
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Cards (22)
Staining procedure give clues to the probable organism being studied
Culture Media
Water-Based solution that do not solidify at the above freezing point.
Liquid Media
Clot-Like consistency at a ordinary room temperature contains agar, allows thickening of media without producing a firm substance.
Semi-Solid Media
Contain solidifying agent such 1.5%-2% agar, firm substance on which cells can form discrete colonies.
Solid Media
Contains chemically-defined substances which are pure organic and inorganic compounds
Synthetic Media
Complex media that contain at least one ingredient THAT IS NOT CHEMICALLY DEFINED.
Non-synthetic media
design for primary isolation of a broad spectrum of microbes, supports the growth of BOTH pathogenic and non-pathogenic organisms.
General purpose media
Contain complex organic substances such as BLOOD, serum (Design to increase the number of the desired microorganisms.
Enrichment media
Contains general nutrients with 5%-10%, blod added to a agar base.
Blood agar
shows complete lysis of red blood cells resulting in complex clearing around colonies
Beta hemolysis
shows complete lysis of red blood cells, producing a greenish discoloration of the blood agar around the colonies.
Alpha hemolysis
show no hemolysis, resulting in no change in the medium.
Gamma hemolysis
A type of nutrient medium that is use for the culture of fastidious organisms such as Haemophilus.
Chocolate agar
contain one or more substances that encourage the growth of the ONLY specific target microorganisms.
Selective media
Used for Isolation of Neissera
Thayer-Martin agar
Used for Isolation of Staphylococcus
Mannitol Salt agar
Promote growth of gram-negative bacteria, and inhibits the growth of gram-positive bacteria through bile of salts.
MacConkey's agar
Selective medium that used to recover mycobacterium tuberculosis made selective by the incorporation of malachite green.
Lowenstein-Jensen medium
Used for Isolation of Fungi
Saboraud's dextrose sugar
Allow the growth of several types of microorganisms.
Differential Media
used for clinical specimens that need to be transported to the laboratory immediately after collection
Transport Media
Media used for specifically for organism that cannot survive in the presence of oxygen and require reduced oxidation potential and other nutrients.
Anaerobic Media