This section defines the samples in the study, the treatments the samples are subjected to, any existing limitations of the samples known at the time of the investigation, and limitations of data-gathering methods. Sources of samples, their quantity, and seasonal nature, if there is any, are also being mentioned in this part of the research paper. Accuracy and precision of equipment used in the study are also stated. Setting the scope and limitations of the study includes giving the time frame for the research in addition to where it will be conducted.