Enzymes - biological catalysts that speed up the rate of reaction by lowering activation energy, they do not get used up in the reaction
lipase :
Breaks down lipids (fats) into fatty acids and glycerol
Produced in pancreas and small intestine.
protease :
Break down protein into amino acids
Produced in stomach, pancreas and small intestine
amylase :
Breaks down starch into maltose (sugars)
Produced in the salivary glands and pancreas
Iodine test for starch from orange brown to blue black
add iodine to the food (starch) it will turn blue - black
Benedict test for glucose (sugar) from blue to yellow / red
heat benedict's reagent in a water bath for two minutes, if glucose present it will turn red
biuret test for protein colour from blue to purple
add biuret reagent to food (protein) is its present it will turn purple
cells - basic building blocks of life
tissue - a group of cells with a similar structure and function working together
organs - group of different tissues working together forming specific functions
organs are organised into organ systems, which work together to form organisms
digestive system is an example of an organ system in which several organs work together to digest and absorb food
as an organ develops cells differentiate to form different types of cells - they become specialised
root hair cells - lots of mitochondria for active transport of minerals
- long projection to increase the surface area to absorb water and minerals
muscle tissue - contract to bring about movement
glandular tissue - produce substances such as enzymes and hormones
epithelial tissue - converts all parts of the body
enzymes are large properties
space within the protein (enzyme) molecule - active site
enzymes work best at optimum temperature pH
lock and key theory - the chemical that reacts is called the substrate (key) and it fits into the enzyme's active site (lock)
high temperature and extreme pH makes enzymes change shape - denaturing
enzyme cannot work once it has been denatured
digestive enzymes are produced by specialised cells in glands and the lining of the gut :
enzymes pass out of the cells into the digestive system
they come in contact with food molecules
they catalyse the breakdown of large insoluble food molecules into smaller soluble molecules
relate knowledge of enzymes to metabolism : enzymes are proteins that help speed up metabolism or chemical reactions in the body
bile is a liquid made in the liver and stored in the gall bladder
bile is an alkali to neutralise hydrochloric acid from the stomach
bile emulsifies fat to form small droplets , increasing the surface area for enzymes to act on
required practical for enzymes:
Put a test tube containing starch solution and a test tube containing amylase into a water bath 37 degrees
After 5 minutes add the amylase solution to starch
every 30 seconds take a drop from the mixture and test it for starch using iodine solution
record how long it takes for the starch to be completely digested
repeated the experiment at different pH values using different buffer solutions
blood is a tissue
blood is made of a liquid called plasma
3 different components in blood :
platelets
red blood cells
white blood cells
digestive enzymes convert food into small soluble molecules that can be absorbed into the bloodstream
products of digestion are used to build new carbohydrates , proteins and lipids. Glucose used in respiration
plasma - transports various chemical substances around the body , such as products of digestion , hormones , urea
red blood cells - contain haemoglobin which binds to oxygen to transport it from the lungs to the tissue and cells which need it for (aerobic) respiration
red blood cells :
do not contain a nucleus (more room for haemoglobin)
very small, fit through tiny capillaries
shaped like discs giving them a large surface area that oxygen can quickly diffuse across
white blood cells:
help protect the body against infections
can change shape
can squeeze out of the blood vessels into the tissues or surround and engulf microorganisms