The smallest unit of life, all living things are composed of cells or cell product
Functions of life (MR SHENG)
Metabolism - undertake essential chemical reactions
Reproduction - produce offspring, either sexually or asexually
Sensitivity - respond to internal and external stimuli
Homeostasis - maintain a stable internal environment
Excretion - removal of waste products
Nutrition - exchange materials with the environment and obtain energy
Growth - move & change shape or size
Water composition
2 hydrogen atoms bonded to 1 oxygen atom (H2O)
Covalent bonding
Unequal sharing of electrons, creating polarity
Water's polarity
Leads to weak associations like hydrogen bonds
Hydrogen bonds
Give water unique physical, cohesive/adhesive, and solvent properties
Surface Area to Volume Ratio
Cells require chemical energy through metabolism and material exchange with the environment, which is linked to surface area and volume respectively. Growing cells aim to maintain a high surface area to volume ratio for optimal survival.
Specialised cells
Increase surface area for efficient material transfer (e.g., intestinal tissue with villi and microvilli)
Small animals
Have a high surface area relative to volume, losing heat rapidly and requiring more energy
Large animals
Have a progressively smaller surface area compared to volume, losing less heat and needing less food
Light Microscopes
Used to look at cells
Electron Microscopes (EM)
Used to look at cells
Transmission EM
Uses transmitted electrons (electrons that are passing through the sample) to create an image
Scanning EM
Creates an image by detecting reflected or knocked-off electrons
Advantages of Light Microscopes
Cheap
Portable
Produces colour images
Different stains can be used to highlights different features
Can sometimes use live materials
Disadvantages of Light Microscopes
Material has to be transparent (thin or sliced into thin sections
Low resolution
Maximum magnification is 1000X to 1500X
Advantages of Electron Microscopes
High resolution
High magnifications (x200 000)
SEM can produce 3D images
Disadvantages of Electron Microscopes
Expensive equipment
Large equipment
Requires highly toxic chemicals for processing
Time consuming & requires skill
Produces B&W images
Materials must be dead
Magnification
Calculation: Image size (with rule) / Actual size (according to scale bar)