Practice Questions

• what does glucose do to CRP?


  shuts it off, preventing lac operon transcription
• ferroplasma acidarmanus have no cell wall
• lakes and the deep sea both have organic and inorganic compounds
• plasmids contain antibiotic resistant genes
• lac operon is regulated by induction where the lacl repressor is synthesized in the active state and the inducer (allolactose) inactivates the repressor
• what is the terminal e- acceptor of geoglobus ahangari?

  Fe3+
• what part of the lux operon synthesizes the autoinducer AHL?

  luxl
• RNA 3 increases virulence gene expression
• inactivated CRP prevents growth on lactose
• in negative regulation, what does the repressor bind to?

  the operator
• transposon mutagenesis: mate with plasmid and select for mutants with transposon
• anammox bacteria uses anaerobic respiration, what is the donor, acceptor, and product?

  e- donor: ammonia

  e- acceptor: nitrite

  product: nitrogen gas
• RNA3 is a type of antisense RNA that can activate or inhibit translation
• are microbes the primary producers in soil environments?

  no, plants are
• when does qourum sensing affect gene expression?

  when high cell density
• thermococcous atlanticus is an obligate anaerobe found in the deep sea
• what 2 things can you use to determine if DNA was transferred by horizontal gene transfer?

  G/C ration and codon preference
• luxl synthesizes AHP, what is its purpose?

  autoinducer, activates transcription
• what are the primary producers in lakes what do they have in common with producers in the deep sea?

  cyanobacteria, use CO2 for carbon source
• geoglobus is an acetate sink and motile coccus
• AgrA regulates RNA 3 and RNA 2
• what is the significance of the lacA gene?

  end of operon, not required for growth
• what bacteria dominates in anerobic sulfate limiting conditions?

  methanogens
• are extracellular cellulases made in aerobic or anaerobic conditions?

  aerobic
• is the cellusome made in aerobic or anaerobic conditions?

  anaerobic
• does acl-B1 or pelagibacter use bacteriarhodopsin?

  acl-b1
• does acl-b1 or pelagibacter use proteorhodopsin?

  pelagibacter
• what are the primary producers in the ocean?

  cyanobacteria (photoautotrophs)
• what is the most common replication error?

  base substitution
• what is the role of cas9 in crispr?

  binds with guide RNA and removes viral DNA
• plasmids can direct their own replication using host machinery