Describe how to carry out a practical to estimate the number of stomata on a leaf. Include the maths for a circular field of view and a square field of view
1) Use nail varnish to cover an area of the leaf. Allow the nail varnish to dry, then peel it off and place it on a microscope slide.
2) Place the slide onto the microscope stage and bring the image into focus.
3) Count the number of stomata in the field of view.
4) Calculate the area of the field of view (πr2 for a circle, width x length for a square).
5) Divide the number of stomata seen by the area of the field of view. This will tell you the number of stomata per mm2. Multiply this by the area of the leaf for the final estimation.