PMLS#22

    Cards (10)

    • Fungal infections are referred to as mycoses and are further identified by the area of infection and/or the causative organisms.
      • Superficial or cutaneous mycoses: These infect the outermost (dead) layers of the skin and hair, as well as the epidermis, hair follicles and deeper layers of the visible skin.
      • Tinea corporis (ringworm) is an example of this type of infection.
      • Subcutaneous mycoses: These fungal infections occur in the dermis and subcutaneous layers of the skin, and may affect the muscles and tissue layers beneath the skin.
      • Systematic mycoses: Systematic fungal infections may be present in multiple organs and/or areas of the body.
    • Potassium Hydroxide Preparation
      • An examination of the sample under the microscope with a drop or two of 10% to 20% potassium hydroxide (KOH) solution is performed immediately after collection to search for the presence of fungi.
    • A wet mount is a means by which living organisms may be observed under the microscope.
    • Sample Collection Procedures for Detection of Parasites
      • Scabies is a parasitic infection with Sarcoptes scabiei, and this may be diagnosed with a skin scrapping.
    • The pinworm is a small white worm that can infect the colon and rectum of humans.
    • Developed in 1884, the Gram stain is a technique that quickly and inexpensively provides information about the bacteria that are present in a sample.
      • Antibiotic sensitivity testing is performed to determine how effective antimicrobial therapy is against a certain type of bacteria.
      • These automated methods use the minimum inhibitory concentration (MIC) method to determine which antimicrobial agent is the best to use for a specific pathogenic bacteria.
      • Many laboratories use an older, more laborious method of testing known as the disk-diffusion method (also know as the Kirby-Bauer method) for antibiotic sensitivity testing.
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