1.Spread a loopful of organisms in a small area near the edge of the plate in Area 1. Don’t gouge medium.
2. Flame the loop and allow it to cool for 5 seconds. Touching a sterile area of the medium will insure coolness.
3. From the edge of Area 1 make 7 or 8 straight streaks to the opposite side of the plate.
4. Flame the loop again, cool it sufficiently, and cross streak over the last streaks, starting near Area 1.
5. Flame the loop again before putting it aside.