effect of substrate con(catalase and hydrogen peroxide)

Created by

MayaRose

Cards (10)

HYPOTHESIS-an increase of substrate leads to an increase in rate as the enzyme molecules become saturated with the substrate. This is because there are more particles to collide together so the active sites of the enzyme have more to bond with ,forming more enzyme substrate complexes.
Substrate concentration will decrease over time (if no new substrate is added)
The rate of reaction will therefore decrease over time
Apparatus:
.Catalase solution at five different concentrations (enzyme)
Hydrogen peroxide solution (substrate)
Buffer solution (to keep the pH constant)
Boiling tube
Bung and delivery tube
Measuring cylinder
Water trough
Stopwatch
what solution is used to mantain a constant ph?
buffer solution
Add a set volume of hydrogen peroxide solution to a boiling tube
Add a set volume of buffer solution to the same boiling tube
Invert a full measuring cylinder into a trough of water(tray)
Place the end of the delivery tube into the open end of the measuring cylinder and attach the other end to a bung
Add a set volume of one concentration of catalase to the boiling tube and quickly place the bung into the boiling tube
Record the volume of oxygen collected in the measuring cylinder by the water displaced every 10 seconds for 60 seconds
Repeat the experiment twice more and calculate the average volume of oxygen produced at each 10 second interval
Repeat the whole experiment for the different concentrations of catalase
Plot the average volume of gas produced against time for each concentration
Compare the initial rate of reaction of each of the concentrations
conclusion
As the concentration of catalase increases the volume of oxygen produced would increase
This is because there would be more available active sites for hydrogen peroxide to use
The volume of oxygen would plateau out after the initial rate of reaction due to the substrate decreasing, having been converted into the product (oxygen)
What was the control variable
substrate conc(hydrogen peroxide solution)
what were we changing in the investigation
Enzyme catalase volume and concentration
DV-rate of reaction(displacement of water by gas)