HSA

• What are the 2 main functions of Human Serum Albumin.

  1. Transport of small and hydrophobic molecules
  2. Osmotic swelling pressure of blood plasma
• Where is Human Serum Albumin produced.

  Liver.
• How many domains does Human serum albumin have.

  3.
• Which AA forms the disulphide bonds.

  Cysteine
• How are the domains split up.

  LSL double loops.
• How are alpha helixes packed.

  Pairwise with hydrophobic residues on the inside.
• What molecules does human serum albumin transport.

  
  

  1. Ions
  2. Fatty Acids
  3. Hormones
  4. Drugs
  5. Carboxylic acids
• How is hormone release affected when combined with HSA.

  Slower.
• Which part of HSA is responsible for Cu 2+ and Ni 2+ transport.

  N-terminal.
• Which molecules do the cystesine residues transport.

  
  

  1. Cadmium
  2. Gold
  3. Silver
  4. Mercury
• What happens upon FA binding.

  
  

  1. Conformational change
  2. FA in hydrophobic residues
• Is serum albumin present in plasma

  Yes
• What does a forest plot do

  Allows you to compare the effects of a drug across different studies. Each study is plotted and the odds ratio calculated.
• What is OR in a forest plot

  This is the odds ratio. It compares the individual study to a control to see which is better.
• What does OR = 1 in a forest plot mean

  That the drug has no effect compared to the control
• What does OR > 1 mean in a forest plot

  The test produced better results than the control
• What does OR < 1 mean in a forest plot

  The control produces better results than the test
• What is the diamond in a forest plot

  It is the combined data of all the studies shown.
• What type of plot is this

  A forest plot

  
• What is the Mr of HSA

  66,500
• What is the PI

  It is the isoelectric point.
• What is the isoelectric point of HSA

  5.67
• What is the isoelectric point

  The pH at which the molecule has no net charge because it is dipolar.
• What type of FA does HSA transport

  long chain
• What type of carboxylic acids does albumin transport

  Heterocyclic and aromatic
• What is a heterocyclic molecule


  A molecule which has an element other than carbon and hydrogen in the aromatic ring
• Which cysteine residue is involved in transport for HSA

  34
• How many alpha helixes surround the FA in HSA

  3 or 4
• What type of protein is HSA

  Globular