Hb

• How many subunits does Hb have.

  4
• How many oxygen atoms can Hb bind to.

  4.
• Is Hb extracellular or intracellular.

  Extracellular.
• What sucstances does Hb transport.

  1. O2
  2. H+
  3. CO2
• 3 Features of deoxyHb.

  1. Dissolves in urea to form dimers [A1B1, A2B2].
  2. No contact between identical chains.
  3. Strong contact between stable dimers.
• What type of bonding is Oxygen to Hb.

  Cooperative.
• Which has higher affinity for oxygen, Mb or Hb at low partial pressures.

  Mb.
• What are the 3 molecules involved in allosteric regulation.

  1 . H+

  2. CO2

  3. 2,3 Biphosphoglycertate
• What are allosteric regulators. What do they do.

  Groups bonded distanly from Heam. Weaken affinity for oxygen.
• Descrbie the Bohr Effect.

  1. Lactic acid produced from anaerobic respiration
  2. Dissolution of CO2 increases [H+]
  3. Curve shifts to RHS
• Describe the role of 2,3-BPG in oxygen affinity.

  1. Produced via glyclosis using Oxygen.
  2. Sits between B subunits in [DEOXY]
  3. Shifts to RHS.
• what is P50 for Mb

  26.6 mm Hg
• What is Kd for Hb

  26.6 mm Hg
• Describe which curves are what

  The left is 3 types of transport using haem groups [Hb Mb, foetal Hb].

  The right is the effect of temperature on Hb.

  A) High temrpeature

  B) Hb

  C) Mb

  D) foetal Hb