Microscopy

• Light microscopes


  Use visible light and magnifying lenses to observe small objects.

  Positive: Can observe sub-cellular structures

  Limitation: lower magnification and resolution
• Electron microscopes


  Use a beam of electrons in a vacuum with a wavelength of less than 1 nm to visualise the specimen.

  Positive: x500,000 magnification, high resolution

  Negative: Destroys the sample
• Magnification = Image size /Actual size